WebOct 30, 2024 · F, Counts of CTb 488-positive cells in anterior, medial, and posterior IC. More CTb 488-positive cells were observed in anterior IC than medial or posterior IC (p < 0.0001); there was no effect of conspecific stress on the number of CTb 488-positive cells at any ROI. G, Counts of Fos-positive cells in each IC region. WebJul 13, 2024 · P4 mice were injected with anterograde tracers (CTB-488 and CTB-594, Thermo Fisher Scientific (C-22841, C-22842)) in the left and right eyes, respectively. PSVue ICV injections were also performed as …
Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 488
WebFeb 12, 2014 · The CTB conjugated to the dye AlexaFluor-488 or AlexaFluor-555 was separately injected into the ventrolateral PAG and the posterior PVT of the same mice (Fig. 1A,B). A substantial number of CeL neurons were labeled by CTB injected into the PAG, whereas fewer were labeled by CTB injected into the PVT, indicating that CeL neurons … WebFigure Legend Snippet: a Retrograde labelling by CTB-488 microinjection in the LSv resulted in prominent expressions in the ventral subiculum (VS) of the hippocampal formation in the ipsilateral brain. Scale bar, 1000 μm. Inset shows enlarged ventral subiculum. Scale bar, 200 μm. b Anterograde tracing by injection of AAV9-CamIIKα … rcp abbott o\u0027rourke
Distinct populations of cortical pyramidal neurons mediate
WebDec 7, 2015 · The CTB 594 labels from the DVC and the CTB 488 labels from the ARC were detected in several brain regions, including the bilateral medial to caudal area of PVN (Fig. 1e, f). The distribution of labeled neurons was … WebAug 2, 2014 · Figure 1 shows photomicrograph examples of CTb-488 injected into the caudal PBN. Images such as these were used to create accurate reconstructions of the location and extent of tracer injections . Each panel contains a medial and lateral view of a Neurolucida 3D reconstruction of CTb-488 injected into the PBN of an individual mouse. WebOct 12, 2024 · To validate and quantify the NAc-projecting ensembles in the VTA, the green retrograde tracer (cholera toxin B subunit, CTB-488) was injected into the NAc of mice, and the tagged ensembles in the VTA were analyzed (Fig. 1c, d). The percentage of retrogradely labeled cells in Mor-Ens was higher than that in Sal-Ens (Fig. 1e). how to speak asian